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一种从人类胚胎干细胞中生成脑器官的静态自导方法
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
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一种从人类胚胎干细胞中生成脑器官的静态自导方法

DOI:
10.3791/60379-v

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08:30 min

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March 04, 2020

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, , , , ,
1Department of Pathology,Yale University School of Medicine, 2Stem Cell Center,Yale University School of Medicine

Capitoli

  • 00:05Introduction
  • 00:53Stem Cell Maintenance
  • 03:03Dissociation of the hESCs for Organoid Culture
  • 04:27Generation of Organoids
  • 06:06Results: Overview of Organoid Growth Conditions and Morphology
  • 07:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议是作为一种以简化、低成本的方式生产脑器官的方法，没有外源性生长因子或基底膜基质，同时仍然保持脑细胞类型的多样性和细胞组织的许多特征。

Tags

Keywords: Brain OrganoidsHuman Embryonic Stem CellsNeurodevelopmental DisordersToxic InsultsBrain CancerReproducibleSimple WorkflowCost-effectiveMatrixH9 HESCsMTeSR1 MediaLow Oxygen IncubatorProtease SolutionPassaging

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