/

/

生物应用近红外发光金纳米簇的合成

JoVE Journal
Bioingegneria

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Bioingegneria

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Please note that all translations are automatically generated. Click here for the English version.

生物应用近红外发光金纳米簇的合成

DOI:

10.3791/60388-v

•

09:11 min

•

March 22, 2020

•

Goutam Pramanik, Alena Keprova, Jan Valenta, Vaclav Bocan, Klaudia Kvaková⁴, Lenka Libusova, Petr Cigler

¹Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, ²Department of Chemical Physics and Optics, Faculty of Mathematics and Physics,Charles University, ³Department of Cell Biology, Faculty of Science,Charles University, ⁴First Faculty of Medicine,Charles University

Capitoli

00:05Introduction
01:10Preparation of Near-infrared Emitting AuNCs
02:13Conjugation of TPP on the Surface of 1
02:55AuNC Internalization into HeLa Cells
03:55Flow Cytometry Analysis
05:26CLSM Imaging of Live HeLa Cells Labeled with 2
07:15Results: Gold Nanoclusters inside HeLa Cells
08:23Conclusion

Summary

Traduzione automatica

介绍了一种可靠且易于重现的方法，用于制备可功能的近红外发光金纳米簇，并通过流细胞学和共聚焦激光扫描显微镜直接检测HeLa细胞内部。

Tags

Keywords: Gold Nanoclusters Near-infrared Emitting Photoluminescent Thiol-functionalized Ligand Amine-functionalized Ligand Biosensing Bio-imaging Ultrafiltration Polyethylene Glycol EDC HCL Cell Culture

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

单层的合成，装配和表征蛋白质单分子膜的电化学保护的金纳米粒子薄膜

黄金Nanostar合成银种子介导的生长方法

宽带超声医疗成像应用，光学检测仪

就地液体生物组件的 TEM

荧光淬一个脂质体包封的近红外荧光团的作为一个工具<em>在体内</em>光学成像

基于角蛋白纳米纤维生物医学工程合成

在脉冲激光激发金纳米粒子集成光电响应脂质体及其微泡空化的测量的合成

从混合三维培养使用近红外Photoimmunotherapy技术选择性细胞消除

硅纳米线场效应晶体管的研制化学生物传感与应用

RGD功能化水凝胶作为治疗应用的工具合成

Read Article