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Immunologia e infezioni

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利用流细胞测定人类白细胞测定阿斯珀吉鲁斯紫锥体烟曲的噬菌体

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

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利用流细胞测定人类白细胞测定阿斯珀吉鲁斯紫锥体烟曲的噬菌体

DOI:

10.3791/60397-v

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09:43 min

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December 07, 2019

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Susann Hartung², Christopher Rauh, Sarah Böttcher, Mai Thi Ngoc Hoang², Susanne Jahreis², Silke Rummler, Andreas Hochhaus, Marie von Lilienfeld-Toal²

¹Infections in Hematology and Oncology,Leibniz Institute for Infection Biology and Natural Product Research, ²Department for Hematology and Medical Oncology,Jena University Hospital, ³Institute for Transfusion Medicine,Jena University Hospital

Capitoli

00:04Titolo
00:53Preparation of Aspergillus fumigatus Conidia
02:26Preparation of Human Primary Leukocytes
03:22Phagocytosis Assay
04:01Antibody Staining
05:58Flow Cytometry
07:24Results: Measuring Phagocytosis
08:58Conclusion

Summary

Traduzione automatica

该协议提供了一种快速可靠的方法，用流动细胞测定人类原发性噬菌体定量测量阿苯甲基的邻苯二甲苯，并区分锥体从单纯的粘附到白细胞的邻形图。

Tags

Keywords: Phagocytosis Aspergillus Fumigatus Conidia Human Leukocytes Flow Cytometry Cell Markers Neutrophils Monocytes Mucorales Red Blood Cells FITC Labeling RPMI Medium Buffy Coat EL Buffer Centrifugation

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