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Capitoli
- 00:05Titolo
- 01:09Day 1: Inoculation and Acclimation of Cultures
- 01:49Day 2: Generation of Mutants in Parallel Cultures
- 03:27Day 3: Plating Cultures on Selective and Non-selective Agar Plates
- 04:48Day 4 and Day 5
- 05:48Results: Analysis of the Fluctuation Assay
- 06:47Conclusion
Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.
