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Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection
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Biologia
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JoVE Journal Biologia
Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

DOI:
10.3791/60411-v

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06:27 min

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February 06, 2020

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, , , , ,
1Department of Pharmacology, The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy,Harbin Medical University, 2Institute of Metabolic Disease,Heilongjiang Academy of Medical Science, 3Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine,Dentistry and Health Sciences University of Melbourne

Capitoli

  • 00:04Titolo
  • 01:17Parabiosis
  • 02:33Circulation Chimerism Validation
  • 04:13Results: Representative Changes in Blood Glucose Levels in Parabionts after Glucose Injection
  • 05:52Conclusion

Summary

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Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts.

Tags

Keywords: ParabiosisChimerismGlucose InjectionTail VeinBlood SharingAnesthesiaSuturingPost-operative CareVerification Method

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