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Isolasjon og utvidelse av cytotoktoktokin-indusert killer T-celler for kreftbehandling
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1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital
Capitoli
- 00:04Introduction
- 00:50Immunophenotyping for Assessment of CIK Cells
- 02:14CD Marker Recognition
- 04:12Cytotoxic Assay
- 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
- 10:10Conclusion
Her presenterer vi en protokoll for å utføre isolasjon og utvidelse av perifert blod mononukleære celler-avledet cytokin-indusert CD3+CD56+ killer celler og illustrere deres cytotoksiskitet effekt mot hematologiske og solide kreftceller ved hjelp av en in vitro diagnose flyt cytometri system.
