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Aislamiento y expansión de células T asesinas inducidas por citoquinas citotóxicas para el tratamiento del cáncer
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1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital
Capitoli
- 00:04Introduction
- 00:50Immunophenotyping for Assessment of CIK Cells
- 02:14CD Marker Recognition
- 04:12Cytotoxic Assay
- 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
- 10:10Conclusion
Aquí, presentamos un protocolo para realizar el aislamiento y expansión de células mononucleares de sangre periférica-inducidas por citoquinas CD3+CD56+ células asesinas e ilustrar su efecto de citotoxicidad contra las células cancerosas hematológicas y sólidas mediante el uso de un sistema de citometría de flujo de diagnóstico in vitro.
