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Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
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Capitoli
- 00:05Titolo
- 01:01Identification of SSHHPS in the Host Genome Using BLAST
- 01:49Design and Preparation of Protease Substrates
- 04:07Continuous and Discontinuous Enzyme Assays
- 06:02Docking Substrate Peptides to the VEEV-nsP2 Cysteine Protease
- 08:27Results: SSHHPS Analysis
- 09:54Conclusion
We present a general protocol for identifying short stretches of homologous host-pathogen protein sequences (SSHHPS) embedded in the viral polyprotein. SSHHPS are recognized by viral proteases and direct the targeted destruction of specific host proteins by several Group IV viruses.
