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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
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Capitoli
- 00:04Titolo
- 01:00Preparation of Adhesive Polystyrene Bead
- 02:54Isolation of Embryo Blastomere
- 05:00Reconstitution of Contact Patterns with Blastomere and Bead
- 05:57Results: Analysis of Cell Division Orientation
- 07:04Conclusion
Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.
