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Isolation og kultur af oculomotor, Trochlear, og spinal motor neuroner fra prænatal ISLMN: gfp Transgene mus
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1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Capitoli
- 00:05Titolo
- 01:17Ventral Midbrain and Spinal Cord Dissection
- 06:10Tissue Dissociation
- 07:23Culture of Purified Primary Motor Neurons
- 07:59Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
- 09:34Conclusion
Dette arbejde præsenterer en protokol til at give homogene cellekulturer af primære oculomotor, trochlear, og spinal motoriske neuroner. Disse kulturer kan anvendes til komparative analyser af de morfologiske, cellulære, molekylære og elektrofysiologiske egenskaber af okulære og spinal motoriske neuroner.
