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Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations
JoVE Journal
Biologia
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JoVE Journal Biologia
Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations
DOI:

04:32 min

May 31, 2020

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Capitoli

  • 00:04Introduction
  • 00:36Fluorescence-Activated Cell Sorting (FACS) Gating Strategy Setup and Analysis
  • 01:49FACS Sorting and Cell Collection
  • 02:55Results: Representative Live Coral Cell Gating
  • 03:59Conclusion

Summary

Traduzione automatica

Corals create biodiverse ecosystems important for both humans and marine organisms. However, we still do not understand the full potential and function of many coral cells. Here, we present a protocol developed for the isolation, labeling, and separation of stony coral cell populations.

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