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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
JoVE Journal
Biochimica
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JoVE Journal Biochimica
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

DOI:
10.3791/60449-v

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07:37 min

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January 09, 2020

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, ,
1Biodynamics Research Center (BDR), RIKEN, 2Research Center,Misr International University

Capitoli

  • 00:04Titolo
  • 01:06Cell Culture
  • 01:44Drug Treatment
  • 02:33Raman Spectral Imaging and Spectral Processing
  • 03:29Single-cell Sampling Set-up and Procedures, and Mass Spectrometry Measurements
  • 05:34Results: Drug-treated and Untreated Cells
  • 06:44Conclusion

Summary

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This protocol presents an integrated Raman spectroscopy-mass spectrometry (MS) platform that is capable of achieving single-cell resolution. Raman spectroscopy can be used to study cellular response to drugs, while MS can be used for targeted and quantitative analysis of drug uptake and metabolism.

Tags

Raman SpectroscopyMass SpectrometrySingle-cell AnalysisDrug UptakeMetabolismCellular HeterogeneityCancer ResistanceMetabolomicsSpectral ImagingMicromanipulatorCell CultureTamoxifenDMSOSpectrophotometer CalibrationMicro-chamber

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