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一个集成拉曼光谱和质谱平台，用于研究单细胞药物接受、代谢和效果
JoVE Journal
Biochimica
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JoVE Journal Biochimica
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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一个集成拉曼光谱和质谱平台，用于研究单细胞药物接受、代谢和效果

DOI:
10.3791/60449-v

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07:37 min

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January 09, 2020

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, ,
1Biodynamics Research Center (BDR), RIKEN, 2Research Center,Misr International University

Capitoli

  • 00:04Titolo
  • 01:06Cell Culture
  • 01:44Drug Treatment
  • 02:33Raman Spectral Imaging and Spectral Processing
  • 03:29Single-cell Sampling Set-up and Procedures, and Mass Spectrometry Measurements
  • 05:34Results: Drug-treated and Untreated Cells
  • 06:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议提供了一个集成的拉曼光谱-质谱（MS）平台，能够实现单细胞分辨率。拉曼光谱可用于研究细胞对药物的反应，而MS可用于药物获取和代谢的靶向和定量分析。

Tags

Raman SpectroscopyMass SpectrometrySingle-cell AnalysisDrug UptakeMetabolismCellular HeterogeneityCancer ResistanceMetabolomicsSpectral ImagingMicromanipulatorCell CultureTamoxifenDMSOSpectrophotometer CalibrationMicro-chamber

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