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使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造
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Biologia
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JoVE Journal Biologia
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
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使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造

DOI:
10.3791/60451-v

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08:25 min

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October 31, 2019

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, , , ,
1Institute of Molecular Plant Sciences, School of Biological Sciences,University of Edinburgh, 2Centre for Synthetic and Systems Biology,University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences,University of Edinburgh

Capitoli

  • 00:04Titolo
  • 01:11Construction of Level T Assemblies
  • 01:43Generation of Mutants by Conjugation
  • 06:46Results: S. elongatus UTEX 2973 and Synechocystis PCC 6803
  • 08:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出一个协议，描述如何使用CyanoGate模块化克隆工具包组装自我复制载体，ii） 通过结合将载体引入蓝藻宿主，iii） 使用板读取器或流式细胞仪。

Tags

Genetic ModificationCyanobacteriaConjugationCyanoGateModular CloningSynthetic BiologyRenewable BiotechnologyTriparental MatingTransformationSynechocystisSynechococcusE. ColiHelper StrainPlasmids

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