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Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
JoVE Journal
Biologia
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JoVE Journal Biologia
Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

DOI:
10.3791/60458-v

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09:50 min

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October 18, 2019

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, , , ,
1Department of Biological Sciences,University of Texas at Dallas, 2Department of Urology,University of Texas Southwestern Medical Center

Capitoli

  • 00:05Titolo
  • 01:17Preparation of Reagents and Fume Hood
  • 01:51Tissue De-paraffinization and Rehydration
  • 02:50Hybridization and Counter-staining
  • 07:26Results: Detection of Tissue-Resident Bacteria in Bladder Biopsies
  • 08:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol is for the unbiased detection of tissue-associated bacteria in patient biopsies by 16S rRNA in situ hybridization and confocal microscopy.

Tags

Keywords: 16S RRNAFluorescence In Situ HybridizationBladder BiopsyBacterial DetectionUnbiased Bacterial IdentificationRecurrent Urinary Tract InfectionBacterial PopulationsCoverslip PlacementImaging

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