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16S rRNA 荧光原位杂交检测膀胱活检中的组织驻留细菌
JoVE Journal
Biologia
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JoVE Journal Biologia
Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
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16S rRNA 荧光原位杂交检测膀胱活检中的组织驻留细菌

DOI:
10.3791/60458-v

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09:50 min

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October 18, 2019

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, , , ,
1Department of Biological Sciences,University of Texas at Dallas, 2Department of Urology,University of Texas Southwestern Medical Center

Capitoli

  • 00:05Titolo
  • 01:17Preparation of Reagents and Fume Hood
  • 01:51Tissue De-paraffinization and Rehydration
  • 02:50Hybridization and Counter-staining
  • 07:26Results: Detection of Tissue-Resident Bacteria in Bladder Biopsies
  • 08:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议用于通过16S rRNA原位杂交和共聚焦显微镜对患者活检中组织相关细菌进行无偏见检测。

Tags

Keywords: 16S RRNAFluorescence In Situ HybridizationBladder BiopsyBacterial DetectionUnbiased Bacterial IdentificationRecurrent Urinary Tract InfectionBacterial PopulationsCoverslip PlacementImaging

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