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Kultur af små koloni variant af Pseudomonas aeruginosa og kvantificering af sin Alginat
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

Kultur af små koloni variant af Pseudomonas aeruginosa og kvantificering af sin Alginat

DOI:
10.3791/60466-v

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09:06 min

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February 22, 2020

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, ,
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Capitoli

  • 00:04Introduction
  • 00:49SCV Growth Conditions and Physiological Activation of the Salvage Pathway
  • 01:47Uronic Acid Carbazole Assay
  • 04:36ELISA for Alginate Quantitation
  • 06:34Results: Uronic Acid Carbazole Assay, Anti-Alginate mAb-Based ELISA, and Direct Patient Sample Testing
  • 08:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her beskriver vi en vækst betingelse for at kultur den lille koloni variant af Pseudomonas aeruginosa. Vi beskriver også to separate metoder til påvisning og kvantificering af exopolysaccharid alginat produceret af P. aeruginosa ved hjælp af en traditionel uroonsyre carbazole assay og en alginat-specifik monoklonal antistof (mAb) baseret ELISA.

Tags

Keywords: Pseudomonas AeruginosaSmall Colony VariantSCVAlginateELISAUronic Acid CarbazolePhysiological ActivationSalvage PathwayOD600

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