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利用膜成熟腺细胞聚合培养（MAAC）的人类成熟腺细胞的分离与培养

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

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利用膜成熟腺细胞聚合培养（MAAC）的人类成熟腺细胞的分离与培养

DOI:

10.3791/60485-v

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06:28 min

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February 13, 2020

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Ida Alexandersson, Matthew J. Harms, Jeremie Boucher^2,3

¹Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca, ²The Lundberg Laboratory for Diabetes Research,University of Gothenburg, ³Wallenberg Centre for Molecular and Translational Medicine,University of Gothenburg

Capitoli

00:05Introduction
00:53Human Subcutaneous Adipose Tissue Dissection
01:32Collagenase Treatment
02:21Mature Adipocyte Purification
03:20Mature Adipocyte Packing
03:51Mature Adipocyte Seeding and Maintenance
04:50Results: Representative Mature Adipocyte Aggregate Culture (MAAC) Appearance and Response to Diverse Pharmacological Agonism
05:55Conclusion

Summary

Traduzione automatica

膜成熟脂肪细胞聚集培养（MAAC）是培养成熟人类脂肪细胞的新方法。在这里，我们详细介绍了如何从人类脂肪中分离脂肪细胞，以及如何设置MAAC。

Tags

Keywords: Mature Adipocyte Adipocyte Culture Membrane Mature Adipocyte Aggregate Cultures (MAAC)Adipose Biology Diabetes Obesity Cell Interactions Adipocyte Isolation Adipocyte Suspension Adipocyte Wash Adipocyte Separation

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