Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for <em>Acinetobacter baumannii</em>

Melina B. Cian; Nicole P. Giordano; Joshua A. Mettlach; Keaton  E. Minor; Zachary D. Dalebroux

doi:10.3791/60517

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Immunologia e infezioni

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将葛兰阴性细菌的细胞包络分离到内膜和外膜分数，对阿辛托巴基的培养素进行技术调整

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

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将葛兰阴性细菌的细胞包络分离到内膜和外膜分数，对阿辛托巴基的培养素进行技术调整

DOI:

10.3791/60517-v

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10:24 min

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April 10, 2020

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Melina B. Cian*¹, Nicole P. Giordano*¹, Joshua A. Mettlach*¹, Keaton E. Minor*¹, Zachary D. Dalebroux

¹Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center

Capitoli

00:04Introduction
00:55Preparation of Bacteria for Membrane Extraction
02:18Dissociation of the Outer Membrane and Plasmolysis
03:56Pressurized Homogenization and Lysis
04:35Total Membrane Fractionation
05:34Separating the Dual Membranes
08:00Results: Purity and Quality of Inner and Outer Membrane Fractions
09:36Conclusion

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革兰氏阴性细菌产生两个空间隔离膜。外膜由围皮层和气膜层从内膜分割。分离这些微生物的双层的能力对于了解它们的生理和发病机制至关重要。

将葛兰阴性细菌的细胞包络分离到内膜和外膜分数，对阿辛托巴基的培养素进行技术调整

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