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Gram-negatif Bakteriler için Hücre Zarfının İç ve Dış Membran Fraksiyonlarına Ayrılması Acinetobacter baumannii için Teknik Ayarlamalar
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Capitoli
- 00:04Introduction
- 00:55Preparation of Bacteria for Membrane Extraction
- 02:18Dissociation of the Outer Membrane and Plasmolysis
- 03:56Pressurized Homogenization and Lysis
- 04:35Total Membrane Fractionation
- 05:34Separating the Dual Membranes
- 08:00Results: Purity and Quality of Inner and Outer Membrane Fractions
- 09:36Conclusion
Tags
Keywords:
Cell EnvelopeGram-negative BacteriaInner MembraneOuter MembraneLipidsProteinsSugarsAntibiotic KillingAntimicrobial ResistanceDisease PathogenesisAcinetobacter BaumanniiCell FractionationDetergent-freeSemi-native EnvironmentOptical DensityCentrifugationLysozymeEDTAProtease InhibitorMagnesium ChlorideNuclease
