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Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
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JoVE Journal Immunologia e infezioni
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

DOI:
10.3791/60523-v

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07:07 min

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June 27, 2020

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, , ,
1CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,University of Chinese Academy of Sciences, 2School of Life Science,University of Science and Technology of China, 3State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences,University of Chinese Academy of Sciences

Capitoli

  • 00:04Introduction
  • 00:50Influenza A/Puerto Rico/8/34 H1N1 (PR8) Virus Inoculation
  • 01:52Mediastinal Lymph Node (mLN) and Splenic Lymphocyte Isolation
  • 03:22Polyclonal T Follicular Helper (Tfh) Cell Immunostaining
  • 04:58Results: Representative Tfh Cell and Germinal Center Responses in a Mouse Model of Influenza A Virus Infection
  • 06:27Conclusion

Summary

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This paper describes protocols of evaluating Tfh and GC B response in mouse model of influenza virus infection.

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Keywords: T Follicular Helper CellsGerminal CenterInfluenza A VirusC57BL/6 MicePR8 VirusSpleenMediastinal Lymph NodeCell IsolationBiosafety

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