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Antibiotic Dereplication Using the Antibiotic Resistance Platform
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Antibiotic Dereplication Using the Antibiotic Resistance Platform

Antibiotic Dereplication Using the Antibiotic Resistance Platform

DOI:
10.3791/60536-v

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10:49 min

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October 17, 2019

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, ,
1Department of Biochemistry and Biomedical Sciences, M.G. DeGroote Institute for Infectious Disease Research,McMaster University, 2Department of Molecular and Cellular Biology,University of Guelph

Capitoli

  • 00:04Titolo
  • 00:38ARP/MARP Frozen Stock Library Plate Preparation
  • 01:53Seed Culture and Replication Plate Preparation
  • 04:29Dereplication Plate MHB Overlay and ARP/MARP Library Plate Preparation
  • 06:51Dereplicating using the ARP/MARP
  • 07:59Results: Dereplication Results
  • 09:35Conclusion

Summary

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We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.

Tags

Keywords: Antibiotic DereplicationAntibiotic Resistance PlatformTemplate CustomizationBeta-lactamE. ColiCation Adjusted MHBARPMARP96 Deep Well PlateGlycerolStreptomycesAntibiotic MediumBennett’s AgarSterility Control

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