/
/
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Titolo
- 00:58Liposome Preparation
- 02:14Surface Preparation of Imaging Chamber
- 02:57Liposome Immobilization
- 03:42Image Acquisition and Data Analysis
- 05:21Liposome Size Calibration
- 06:42Results: Liposome Surface Immobilization and Compositional Inhomogeneity
- 08:05Conclusion
This protocol describes the fabrication of liposomes and how these can be immobilized on a surface and imaged individually in a massive parallel manner using fluorescence microscopy. This allows for the quantification of the size and compositional inhomogeneity between single liposomes of the population.
