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Detecting Migration and Infiltration of Neutrophils in Mice
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Biologia
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JoVE Journal Biologia
Detecting Migration and Infiltration of Neutrophils in Mice

Detecting Migration and Infiltration of Neutrophils in Mice

DOI:
10.3791/60543-v

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10:33 min

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February 06, 2020

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, , , , , , ,
1School of Life Sciences,Beijing University of Chinese Medicine, 2State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences,Sun Yat-Sen (Zhongshan) University

Capitoli

  • 00:04Introduction
  • 01:06Air Pouch Assay
  • 02:45Induction of the Adjuvant-induced Arthritis (AA) Mouse Model
  • 04:05Immunohistochemical Staining of Joint Sections
  • 08:56Results: Air Pouch Assay, Adjuvant-induced Arthritis Mouse Model and Immunohistochemical Assay
  • 09:51Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present three methods to assess neutrophil migration and infiltration both in vivo and in vitro. These methods can be used to discover promising therapeutics targeting neutrophil migration.

Tags

Keywords: Neutrophil MigrationAir Pouch AssayAdjuvant-induced ArthritisInflammatory ExudateNeutrophil QuantificationFreund’s AdjuvantMouse ModelInflammation

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