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Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
DOI:

07:30 min

December 07, 2019

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Capitoli

  • 00:04Titolo
  • 00:29Differential Fluorescent Cell Labeling
  • 02:00Cell-Cell Fusion
  • 03:03Fused Cell Enrichment
  • 04:18Cell-Cell Fusion Immunofluorescent Staining and Imaging
  • 05:30Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
  • 06:47Conclusion

Summary

Traduzione automatica

The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

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