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Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells
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Biologia
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JoVE Journal Biologia
Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

DOI:
10.3791/60557-v

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07:47 min

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January 21, 2020

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, , , , ,
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College,Zhejiang Ocean University

Capitoli

  • 00:04Titolo
  • 00:51Intestinal Cell Preparation
  • 02:25Cell Culture
  • 04:17Apoptosis Induction and Detection in A. japonicus Intestinal Cells
  • 06:03Results: Microscopy and Statistical Analysis
  • 07:08Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol provides an easy-to-handle method to culture the intestinal cells from sea cucumber Apostichopus japonicus and is compatible with a variety of widely available tissue samples from marine organisms including Echinodermata, Mollusca, and Crustacea.

Tags

Primary Cell CultureSea CucumberIntestinal CellsApoptosisCell Culture ProtocolMarine InvertebrateTrypsinCell StrainerCentrifugationCell Culture MediumAntibioticsIncubator

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