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利用人类胚胎干细胞的单细胞培养进行高效神经分化

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

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利用人类胚胎干细胞的单细胞培养进行高效神经分化

DOI:

10.3791/60571-v

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11:17 min

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January 18, 2020

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Kilsoo Jeon, Kyeyoon Park, Anton M. Jetten

¹Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences,National Institutes of Health, ²NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Capitoli

00:05Titolo
01:05Adaptation of Colony Type hESCs to Single-cell hESC Culture
04:01Embryoid Body Formation and Differentiation
05:44NPC Expansion and Cryopreservation
06:41Differentiation of NPCs into Dopaminergic Neurons and Astrocytes
08:54Results: In vitro Differentiation of Adapted Single-cell type hESCs
10:34Conclusion

Summary

Traduzione automatica

这里介绍的是人类胚胎干细胞单细胞培养及其随后分化为神经祖细胞的一个方案。该协议简单、可靠、可扩展，适用于药物筛选和再生医学应用。

Tags

Keywords: Human Embryonic Stem Cells Neural Progenitor Cells Single-cell Culture Efficient Differentiation Scalable Culture System Multi-step Differentiation Neural Lineages Basement Membrane Matrix Cell Detachment MTeSR-1 Medium

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