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Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

DOI:
10.3791/60575-v

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07:29 min

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January 29, 2020

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,
1Department of Biological Sciences,Murray State University

Capitoli

  • 00:04Introduction
  • 00:53Embryo Collection and Fixation
  • 03:19Primary and Secondary Antibody Incubation
  • 04:41Embryo Dissection, Deyolking, and Documentation
  • 05:40Results: Whole Mount Immunohistochemistry
  • 06:31Conclusion

Summary

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Here, we present a protocol for fluorescent antibody-mediated detection of proteins in whole preparations of zebrafish embryos and larvae.

Tags

Whole Mount ImmunohistochemistryZebrafish EmbryosLarvaeProtein ExpressionSpatial And TemporalConfocal Microscopy3D RepresentationBiomedical ResearchDisease EtiologyMolecular MechanismsDiagnostic ToolTumor IdentificationSpawningDechorionationFixationPermeabilizationRehydrationMethanol Storage

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