Rivista
/
Biologia
/
一种从小鼠中分离大脑围取体的高输出方法
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A High Output Method to Isolate Cerebral Pericytes from Mouse
Please note that all translations are automatically generated. Click here for the English version.

一种从小鼠中分离大脑围取体的高输出方法

DOI:
10.3791/60588-v

•

06:49 min

•

January 14, 2020

•

, , , , ,
1Laboratory of the Blood Brain Barrier,University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim,Heidelberg University

Capitoli

  • 00:04Titolo
  • 00:44Brain Tissue Recovery and Meninges Removal
  • 01:31Homogenization
  • 02:18Vascular Fraction Isolation
  • 04:32Cerebral Pericyte Proliferation
  • 05:25Results: Representative Cell Morphology, Marker Expression, and Culture Purity
  • 06:25Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了提取鼠脑围细胞的规程。该方案基于无抗生素富集定向围取剂，是体外研究提供高纯度、高产量的宝贵工具，从而减少了实验动物的数量。

Tags

Keywords: Cerebral PericytesPericyte IsolationMouse BrainIn Vitro StudiesCell CultureCell YieldHigh PurityHomogenizationVascular FractionBovine Serum Albumin DextranCell SuspensionFiltration

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/60588?article_type=v

微阵列后探测器（mPADs）：机械力的方法来隔离
check_url/it/60588?article_type=v

双链DNA定量，使用日立F - 7000荧光分光光度计和PicoGreen染料
check_url/it/60588?article_type=v

高通量原位杂交方法表征在胎鼠下泌尿生殖道的基因表达模式
check_url/it/60588?article_type=v

粉末样品的绝对量子产量测量
check_url/it/60588?article_type=v

诱导多能干细胞来源于小鼠的生成
check_url/it/60588?article_type=v

一个在体内交联方法分离蛋白复合物从果蝇胚胎
check_url/it/60588?article_type=v

高分辨率法监测IRF3的磷酸化依赖性激活
check_url/it/60588?article_type=v

胚胎干细胞的跨内细胞质量注射导致嵌合体率增高
check_url/it/60588?article_type=v

小鼠骨髓胰岛移植的一种方法
check_url/it/60588?article_type=v

从兔模型中分离纯化滑液源性间充质干细胞的磁活化细胞分选策略

Read Article