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Dissektion og lipid dråbe farvning af Oenocytter i Drosophila larver
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Biologia
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JoVE Journal Biologia
Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Dissektion og lipid dråbe farvning af Oenocytter i Drosophila larver

DOI:
10.3791/60606-v

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05:37 min

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December 28, 2019

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, , ,
1Sino-French Hoffmann Institute, School of Basic Medical Sciences,Guangzhou Medical University, 2The Second Affiliated Hospital of Guangzhou Medical University, 3The State Key Laboratory of Respiratory Disease,Guangzhou Medical University

Capitoli

  • 00:04Titolo
  • 00:32Egg Laying
  • 01:13Starvation Treatment
  • 02:10Oenocyte Dissection
  • 03:10Lipid Droplet Staining
  • 04:02Mounting and Imaging
  • 04:41Results: Representative Lipid Droplet Staining
  • 05:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

Præsenteret her er detaljerede metoder til dissektion og lipid dråbe farvning af oenocytter i Drosophila larver ved hjælp af bodipy 493/503, en lipid dråbe specifik fluorescerende farvestof.

Tags

OenocyteLipid DropletDrosophilaLarvaeDissectionStainingStarvationMetabolismEpidermisPBSFixation Buffer

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