/

/

监测PD-1-阻断抗体绑定到T细胞派生从外周血滴

JoVE Journal
Medicina

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Medicina

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Please note that all translations are automatically generated. Click here for the English version.

监测PD-1-阻断抗体绑定到T细胞派生从外周血滴

DOI:

10.3791/60608-v

•

06:07 min

•

February 05, 2020

•

Yujiro Naito, Akio Osa, Kentaro Masuhiro, Takashi Hirai, Shohei Koyama, Atsushi Kumanogoh

¹Department of Respiratory Medicine and Clinical Immunology,Osaka University Graduate School of Medicine

Capitoli

00:04Introduction
00:35Whole Blood Sample Preparation and Staining
02:22Flow Cytometric Analysis
03:44Results: Flow Cytometry Analysis
05:15Conclusion

Summary

Traduzione automatica

我们开发了一个简单的流式细胞测定法，用于评估PD-1阻滞抗体与T细胞的结合，只需要癌症患者的一滴外周血。

Tags

Keywords: PD-1-blocking Antibodies T Cells Peripheral Blood Flow Cytometry CD8 T Cells CD4 T Cells PD-1 Human IgG4 Antitumor Effects Immune-related Adverse Events Blood Sample Cell Isolation Cell Staining Data Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

测序外周血中细菌菌群：我们的经验与艾滋病毒感染者

快速点护理依诺肝素的抗凝血剂功效的测定全血中

修复的临界大小的颅骨缺损模型脂肪间充质干细胞的收获Lipoaspirate

高通量顺序酶联免疫吸附试验的急性移植物抗宿主病的生物标志物的验证

人牙髓干细胞的分离，鉴定和比较性恒牙使用两种不同的方法，得出

延时成像的主要癌前乳腺上皮细胞来源于遗传工程小鼠乳腺癌模型

在II-45同基因大鼠模型间皮瘤原位移植和外周免疫细胞监视

无麻醉大鼠尾静脉血采集系统的研究

微电极镀膜法记录从可吸入性中脑动脉的膜电位

分离脂肪衍生的再生细胞用于治疗根治性前列腺切除术后的勃起功能障碍

Read Article