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Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
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JoVE Journal Biochimica
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

DOI:
10.3791/60619-v

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09:11 min

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November 23, 2019

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, , , , , , ,
1Research Program in Molecular and Integrative Biosciences,University of Helsinki, 2Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy,University of Helsinki, 3School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology,University of Leeds

Capitoli

  • 00:04Titolo
  • 00:41Protein Preparation
  • 02:49Compound Preparation
  • 03:44Reagents for the Assay Preparation
  • 05:15Activity Assay for One 96 Well Plate
  • 07:34Results: Color Development, Curve of the TmPPase Percent Activity, and Inhibition Curve
  • 08:46Conclusion

Summary

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Here we present a screening method for membrane-bound pyrophosphatase (from Thermotoga maritima) inhibitors based on the molybdenum blue reaction in a 96 well plate format.

Tags

Keywords: Membrane-bound PyrophosphataseInhibitorsParasitic DiseasesReactivation BufferReaction MixtureLiposomesEnzyme ReactivationD-M-S-OCompound Screening96-well Plate

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