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CryoAPEX-metoden for elektronmikroskopianalyse av membranproteinlokalisering innenfor ultrastrukturelt bevarte celler
JoVE Journal
Biologia
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JoVE Journal Biologia
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
DOI:

11:45 min

February 27, 2020

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Capitoli

  • 00:05Titolo
  • 01:36Transfection, Chemical Fixation and Peroxidase Reaction
  • 03:48High Pressure Freezing
  • 04:46Freeze Substitution
  • 06:23Resin Infiltration and Embedding
  • 08:11Sectioning and TEM Imaging
  • 09:17Results: TEM Images
  • 11:07Conclusion

Summary

Traduzione automatica

Denne protokollen beskriver cryoAPEX-metoden, der et APEX2-merket membranprotein kan lokaliseres ved overføring elektronmikroskopi innenfor optimalt bevart celleultrastruktur.

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