È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics,Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences,Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery,University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University
Capitoli
- 00:04Titolo
- 01:13Islet Preparation
- 05:39Inguinal Subcutaneous White Adipose Tissue (ISWAT) Islet Transplantation
- 08:13Results: Representative Islet Morphology and Function
- 09:24Conclusion
In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.
