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Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

DOI:
10.3791/60681-v

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12:27 min

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January 03, 2020

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, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Capitoli

  • 00:00Titolo
  • 00:44Polymorphonuclear Leukocyte Purification
  • 04:34Cytotoxicity Assay of S. aureus Extracellular Proteins Against Human Polymorphonuclear Leukocytes
  • 06:16S. aureus Cytotoxicity Against Human Polymorhonuclear Leukocytes Following Phagocytosis
  • 08:40Risultati
  • 11:02Conclusion

Summary

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This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

Tags

Keywords: Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

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