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Biochemistry

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JoVE Journal Biochemistry
Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
 

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Article DOI: 10.3791/60686-v • 06:13 min • February 21st, 2020
February 21st, 2020 •
1, 1, 1, 1, 2, 2, 3, 3, 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

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This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.

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Keywords: Stepwise Dosing Label-free Impedance GPCR Assays Throughput Single Transduction Organ-on-chip Cell Seeding Impedance Recording Agonist Mode Serial Addition
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