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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
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JoVE Journal Chimica
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

DOI:
10.3791/60690-v

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05:35 min

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January 17, 2020

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, , ,
1Department of Chemistry and Biochemistry,James Madison University, 2Biophysics Graduate Program,Ohio State University

Capitoli

  • 00:04Titolo
  • 00:55Assay Component Preparation
  • 01:36Sample Preparation, Measurement, and Metal Quantification
  • 03:24Results: Absorbance Spectra and Ni2+ Quantification
  • 04:42Conclusion

Summary

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We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

Tags

Metal LeachingImmobilized Metal Affinity ChromatographyUV-Vis SpectrophotometryHNB ReagentProtein QuantificationBuffer SolutionSpectral AnalysisSample PreparationMetal Concentration Determination

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