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Isolation, Kultur og adipogen induktion af neuralcrest original fedt-afledte stamceller fra periaortisk fedtvæv
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1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
Capitoli
- 00:04Introduction
- 01:24Periaortic Adipose Tissue (PAAT) Dissection
- 02:55Stromal Vascular Cell Fraction (SVCF) Isolation
- 04:51Neural Crest Original Adipose-Derived Stem Cell (NCADSC) Culture
- 06:13NCADSC Adipogenic Induction
- 06:42Results: Representative Adipocyte Differentiation and Characterization
- 07:58Conclusion
Vi præsenterer en protokol for isolation, kultur og adipogen induktion af neurale crest afledte stamceller (NCADSCs) fra det periaortiske fedtvæv af Wnt-1 Cre+/-; Rosa26RFP/+ mus. NCADSCs kan være en let tilgængelig kilde til Adscs til modellering adipogenesis eller lipogenese in vitro.
