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植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定

JoVE Journal
Genetica

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JoVE Journal Genetica

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

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植物病原体分泌效应器中RNA沉默抑制器的筛选和鉴定

DOI:

10.3791/60697-v

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10:19 min

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February 03, 2020

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Jinxia Shi, Yijuan Jia, Di Fang, Shidan He, Peng Zhang², Yushuang Guo, Yongli Qiao

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences,Shanghai Normal University, ²College of Agriculture,Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation,Guizhou Institute of Tobacco Science

Capitoli

00:04Introduction
00:26Preparation of N. benthamiana 16c Plants
02:08Preparation of Agrobacterium Culture for Infiltration
03:44Co-infiltration N. benthamiana Leaves
04:26GFP Imaging Analysis
04:47Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
08:08Results: Infiltrated N. benthamiana 16c Leaves
09:24Conclusion

Summary

Traduzione automatica

在这里，我们提出了一种经过改良的筛选方法，该方法可广泛用于快速筛选植物病原体中的RNA沉默抑制剂。

Tags

Keywords: RNA Silencing Suppressors Plant Pathogens Screening Assay Nicotiana Benthamiana Systemic RNA Silencing Local RNA Silencing Agrobacterium LB Medium Antibiotics MES Acetosyringone

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