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使用亲脂荧光染料的果蝇胚胎运动神经元的逆行追踪

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

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使用亲脂荧光染料的果蝇胚胎运动神经元的逆行追踪

DOI:

10.3791/60716-v

•

08:25 min

•

January 12, 2020

•

Melissa Ana Inal, Kota Banzai, Daichi Kamiyama

¹Department of Cellular Biology,University of Georgia

Capitoli

00:04Titolo
00:49Preparation of Equipment
02:03Embryo Staging
03:10Dissection and Staining
04:14Dye Injection and Confocal Microscopy
06:45Results: Retrograde Labeling of aCC and RP3 Motor Neurons
07:32Conclusion

Summary

Traduzione automatica

我们描述了一种使用亲脂荧光染料逆行追踪果蝇胚胎运动神经元的方法。

Tags

Drosophila Embryonic Motor Neurons Lipophilic Fluorescent Dyes Retrograde Tracing Neuronal Morphogenesis Connectivity Axon Terminal Microinjector Dissection Embryo Collecting Filtration Micropipette Beveling Bleach Dechorionation

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