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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

DOI:
10.3791/60726-v

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00:09 min

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February 18, 2020

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, , , , , , ,
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Department of Biology,College of William and Mary, 3Harvard Medical School,Harvard University, 4Department of Bioengineering,California Institute of Technology, 5Department of Computer Science,College of William and Mary

Capitoli

  • 00:04Introduction
  • 00:34Dissection and Imaging Plate Preparation
  • 01:28Embryo Dissection
  • 05:27Calcium Imaging
  • 07:19Results: Representative Fluorescent Calcium Imaging and Subsequent In Situ Hybridization
  • 08:41Conclusion

Summary

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We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

Tags

Fluorescent Calcium ImagingIn Situ HybridizationNeuronal PrecursorXenopus LaevisSingle Cell AnalysisCalcium ActivityGene ExpressionDissectionCollagenase

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