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Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

DOI:
10.3791/60731-v

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06:25 min

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February 08, 2020

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, , , , ,
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Capitoli

  • 00:05Introduction
  • 00:43Female Whitefly Midgut, Primary Salivary Gland (PSG), Ovary, and Hemolymph Dissection
  • 02:03Tomato Yellow Leaf Curl Virus (TYLCV) Localization
  • 03:40TYLCV Quantification
  • 05:04Results: Representative TYLCV Detection and Quantification
  • 05:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.

Tags

Keywords: BegomovirusesWhiteflyLocalizationQuantificationImmunofluorescenceQuantitative PCRVirus-vector InteractionVirus DetectionVirus TransmissionMicroscopyVirus Quantification

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