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Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

DOI:
10.3791/60744-v

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07:35 min

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February 02, 2020

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, , ,
1Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute,University of Utah School of Medicine

Capitoli

  • 00:05Introduction
  • 00:49Fixing Larvae and Dissecting Tissues Surrounding Liver
  • 02:53Imaging
  • 04:05Image Analysis
  • 06:07Results: Zebrafish Larvae
  • 06:53Conclusion

Summary

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Here we demonstrate a method for quantifying liver size in larval zebrafish, providing a way to assess the effects of genetic and pharmacologic manipulations on liver growth and development.

Tags

Liver SizeLarval ZebrafishBrightfield MicroscopyOrgan QuantificationGenetic ManipulationPharmacologic ManipulationLiver GrowthLiver DevelopmentDissectionIn Situ HybridizationConfocal ImagingEuthanasiaPBSPFASkin RemovalYolk RemovalForcepsGlass Dish

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