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Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
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Genetica
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JoVE Journal Genetica
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

DOI:
10.3791/60749-v

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12:32 min

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December 14, 2019

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, , ,
1Institute of Microbiology,Kiel University

Capitoli

  • 00:00Titolo
  • 01:53Monitoring Plasmid-carrying Bacteria under Various Conditions over Time
  • 10:14Risultati
  • 11:22Conclusion

Summary

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Our experimental approach provides a strategy to follow plasmid abundance and antibiotic resistance over time in bacterial populations.

Tags

PlasmidAntibiotic ResistanceLateral Gene TransferPCRIsothermal AssemblyE. ColiMG1655Experimental EvolutionReplica Plating96-well Plate

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