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Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins
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Capitoli
- 00:04Introduction
- 00:51Preparation for Surgery
- 01:44Surgery
- 04:47In Vitro Recording
- 05:27Results: ChrimsonR is an Effective Red-shifted Opsin for Long-range CRACM Experiments in the Auditory Brainstem
- 06:26Conclusion
Channelrhodopsin-assisted circuit mapping (CRACM) is a precision technique for functional mapping of long-range neuronal projections between anatomically and/or genetically identified groups of neurons. Here, we describe how to utilize CRACM to map auditory brainstem connections, including the use of a red-shifted opsin, ChrimsonR.
