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JoVE Journal Medicine
Taking the Next Step
 

Taking the Next Step: a Neural Coaptation Orthotopic Hind Limb Transplant Model to Maximize Functional Recovery in Rat

Article DOI: 10.3791/60777-v • 14:18 min • August 30th, 2020
August 30th, 2020 •
1,3, 1,2, 1, 1, 1, 1, 1,4, 1, 1, 1, 1
1Comprehensive Transplant Center and Department of Surgery, Feinberg School of Medicine, Northwestern University, 2Department of Surgery, University of Illinois at Chicago, 3Department of Surgery, Tianjin Occupational Diseases Precaution and Therapeutic Hospital, 4Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University

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This protocol presents a robust, reproducible model of vascularized composite allotransplant (VCA) geared toward simultaneous study of immunology and functional recovery. The time invested in meticulous technique in a right mid-thigh hind limb orthotopic transplant with hand sewn vascular anastomoses and neural coaptation yields the ability to study functional recovery.

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Keywords: Limb Transplant Vascularized Composite Allotransplant VCA Microsurgery Neural Coaptation Bone Reattachment Vascular Anastomosis Muscle Reattachment Skin Graft Functional Recovery Rat Model Microsurgical Techniques
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