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结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因
JoVE Journal
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JoVE Journal Ricerca sul cancro
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
DOI:

09:24 min

May 09, 2020

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Capitoli

  • 00:04Introduction
  • 01:02Generation of Inducible Knockdown GCSCs Lines
  • 03:31Generation of Stable Transfected Cell Lines
  • 06:12Sphere Formation Assay
  • 06:54Results: Inducible Knockdown of Clusterin Inhibits GCSC Self-renewal Capacity
  • 08:22Conclusion

Summary

Traduzione automatica

在该协议中,我们提出了一个实验设计,使用条件敲制系统和适应球形分析,以研究聚类对患者衍生GCSC的茎的影响。该协议可以很容易地适应研究在不同类型的CSC的茎相关基因的体外和体内功能。

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