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High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
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High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

DOI:
10.3791/60805-v

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06:41 min

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March 10, 2020

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, ,
1Department of Plant and Microbial Biology,University of California, Berkeley, 2Plant Gene Expression Center,United States Department of Agriculture

Capitoli

  • 00:05Introduction
  • 00:55Preparation of Plant Materials
  • 02:43Bacterial Culture and Preparation of PstT1 Inoculum
  • 03:36Tomato Seedling Flood Method
  • 04:44Results: Phenotypic and Quantitative Screening of Seedling Resistance
  • 06:06Conclusion

Summary

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The seedling flood assay facilitates rapid screening of wild tomato accessions for the resistance to the Pseudomonas syringae bacterium. This assay, used in conjunction with the seedling bacterial growth assay, can assist in further characterizing the underlying resistance to the bacterium, and can be used to screen mapping populations to determine the genetic basis of resistance.

Tags

Keywords: High-throughputResistancePseudomonas Syringae Pv. TomatoTomatoSeedling Flood AssayBacterial PathogenWild AccessionsComplex Genetic BackgroundsFlood InoculationBacterial PathogensTomato SeedlingsSterileStratifyGrowth ChamberCotyledonsTrue LeavesPst T1InoculumOptical Density

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