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Journal / Genetics / 유전자 변형 마우스의 고효율 생산을 위한 동결 해동 배아…
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Genetics

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JoVE Journal Genetics
유전자 변형 마우스의 고효율 생산을 위한 동결 해동 배아 사용
 
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유전자 변형 마우스의 고효율 생산을 위한 동결 해동 배아 사용

Article DOI: 10.3791/60808-v • 06:46 min • April 2nd, 2020
April 2nd, 2020 •
*1,2,3, *4,5, 6,7, 8,9,10, 8,11, 3, 8,9,10,12,13, 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
* These authors contributed equally

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여기에서, 우리는 1 세포 배아의 동결 보존을 위한 수정된 방법 뿐만 아니라 유전자 변형 마우스의 효율적인 생성을 위한 동결 해동 된 배아 및 전기 천공의 사용을 결합하는 프로토콜을 제시한다.

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유전학 문제 158 CRISPR/Cas9 1 세포 배아 동결 보존 전기 천공 유전자 변형 마우스 GM 게놈 편집 FBS
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