Rivista
/
Biochimica
/
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

DOI:
10.3791/60813-v

•

08:02 min

•

March 12, 2020

•

, , , ,
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, 2Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Capitoli

  • 00:05Introduction
  • 00:49Colon Sample Preparation
  • 03:12Immunohistochemistry
  • 06:07Results: Estrogen Receptor Localization in the TNBS-induced Crohn s Disease
  • 07:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

The protocol presents a complete validated TNBS-induced murine model of Crohn's disease and methods for visualization of estrogen receptors by immunohistochemistry using immunofluorescence of formalin-fixed colon sections embedded in paraffin.

Tags

Estrogen ReceptorImmunofluorescenceTNBS-induced Crohn’s DiseaseMouse ColonParaffin EmbeddingMicrotome SectioningDeparaffinization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/60813?article_type=v

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
check_url/it/60813?article_type=v

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
check_url/it/60813?article_type=v

Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy
check_url/it/60813?article_type=v

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
check_url/it/60813?article_type=v

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
check_url/it/60813?article_type=v

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
check_url/it/60813?article_type=v

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
check_url/it/60813?article_type=v

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice
check_url/it/60813?article_type=v

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
check_url/it/60813?article_type=v

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Read Article